endogenous gdnf Search Results


b31 a3 genomic dna  (Thermo Fisher)
99
Thermo Fisher b31 a3 genomic dna
The in-frame deletion of bb0318 is nonpolar and has no effect on spirochete growth in vitro. (A) Schematic representation of the wild-type (WT) and Δbb0318 loci on the chromosome. The sequence of the BB0318 open reading frame was replaced directly in frame with the aadA antibiotic resistance cassette in order to prevent a polar effect on the expression of the surrounding genes. Dashed lines mark deletion boundaries; primer numbers and small arrows indicate the locations of primers used to verify the mutant construct. (B) PCR analysis of the bb0318 mutant clone. <t>Genomic</t> <t>DNAs</t> from WT-background spirochetes and Δbb0318 spirochetes were used as the templates for PCR analyses (indicated above the gel). The primer pairs used to amplify specific <t>DNA</t> sequences correspond to the labels in panel A and are given beneath the gel. DNA ladder fragment sizes (in base pairs) are given on the left. (C) qRT-PCR analysis of genes surrounding bb0318 in mutant and complemented clones. Spirochete RNA was isolated from log-phase-grown WT, mutant, and complemented clones, and the expression of the recA, bb0319, bb0318, bb0317, and bb0316 genes was quantified by reverse transcriptase qPCR. The data are expressed as the number of mRNA transcripts per number of recA transcripts and are averages for three biological replicates. (D) Analysis of the growth of WT, Δbb0318/pBSV2G, and Δbb0318/pBSV2G bb0318 spirochetes. Clones were grown in 5-ml biological triplicate cultures and were enumerated by dark-field microscopy every 24 h.
B31 A3 Genomic Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/endogenous+gdnf/pmc05067747-105-23-31?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
b31 a3 genomic dna - by Bioz Stars, 2026-08
99/100 stars
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endogenous gdnf  (R&D Systems)
92
R&D Systems endogenous gdnf
The in-frame deletion of bb0318 is nonpolar and has no effect on spirochete growth in vitro. (A) Schematic representation of the wild-type (WT) and Δbb0318 loci on the chromosome. The sequence of the BB0318 open reading frame was replaced directly in frame with the aadA antibiotic resistance cassette in order to prevent a polar effect on the expression of the surrounding genes. Dashed lines mark deletion boundaries; primer numbers and small arrows indicate the locations of primers used to verify the mutant construct. (B) PCR analysis of the bb0318 mutant clone. <t>Genomic</t> <t>DNAs</t> from WT-background spirochetes and Δbb0318 spirochetes were used as the templates for PCR analyses (indicated above the gel). The primer pairs used to amplify specific <t>DNA</t> sequences correspond to the labels in panel A and are given beneath the gel. DNA ladder fragment sizes (in base pairs) are given on the left. (C) qRT-PCR analysis of genes surrounding bb0318 in mutant and complemented clones. Spirochete RNA was isolated from log-phase-grown WT, mutant, and complemented clones, and the expression of the recA, bb0319, bb0318, bb0317, and bb0316 genes was quantified by reverse transcriptase qPCR. The data are expressed as the number of mRNA transcripts per number of recA transcripts and are averages for three biological replicates. (D) Analysis of the growth of WT, Δbb0318/pBSV2G, and Δbb0318/pBSV2G bb0318 spirochetes. Clones were grown in 5-ml biological triplicate cultures and were enumerated by dark-field microscopy every 24 h.
Endogenous Gdnf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/endogenous+gdnf/bio_rxiv__2024__02__07__579396-248-2-13?v=R%26D+Systems
Average 92 stars, based on 1 article reviews
endogenous gdnf - by Bioz Stars, 2026-08
92/100 stars
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gdnf  (Aladdin Scientific Corporation)
N/A
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Image Search Results


The in-frame deletion of bb0318 is nonpolar and has no effect on spirochete growth in vitro. (A) Schematic representation of the wild-type (WT) and Δbb0318 loci on the chromosome. The sequence of the BB0318 open reading frame was replaced directly in frame with the aadA antibiotic resistance cassette in order to prevent a polar effect on the expression of the surrounding genes. Dashed lines mark deletion boundaries; primer numbers and small arrows indicate the locations of primers used to verify the mutant construct. (B) PCR analysis of the bb0318 mutant clone. Genomic DNAs from WT-background spirochetes and Δbb0318 spirochetes were used as the templates for PCR analyses (indicated above the gel). The primer pairs used to amplify specific DNA sequences correspond to the labels in panel A and are given beneath the gel. DNA ladder fragment sizes (in base pairs) are given on the left. (C) qRT-PCR analysis of genes surrounding bb0318 in mutant and complemented clones. Spirochete RNA was isolated from log-phase-grown WT, mutant, and complemented clones, and the expression of the recA, bb0319, bb0318, bb0317, and bb0316 genes was quantified by reverse transcriptase qPCR. The data are expressed as the number of mRNA transcripts per number of recA transcripts and are averages for three biological replicates. (D) Analysis of the growth of WT, Δbb0318/pBSV2G, and Δbb0318/pBSV2G bb0318 spirochetes. Clones were grown in 5-ml biological triplicate cultures and were enumerated by dark-field microscopy every 24 h.

Journal: Infection and Immunity

Article Title: Gene bb0318 Is Critical for the Oxidative Stress Response and Infectivity of Borrelia burgdorferi

doi: 10.1128/IAI.00430-16

Figure Lengend Snippet: The in-frame deletion of bb0318 is nonpolar and has no effect on spirochete growth in vitro. (A) Schematic representation of the wild-type (WT) and Δbb0318 loci on the chromosome. The sequence of the BB0318 open reading frame was replaced directly in frame with the aadA antibiotic resistance cassette in order to prevent a polar effect on the expression of the surrounding genes. Dashed lines mark deletion boundaries; primer numbers and small arrows indicate the locations of primers used to verify the mutant construct. (B) PCR analysis of the bb0318 mutant clone. Genomic DNAs from WT-background spirochetes and Δbb0318 spirochetes were used as the templates for PCR analyses (indicated above the gel). The primer pairs used to amplify specific DNA sequences correspond to the labels in panel A and are given beneath the gel. DNA ladder fragment sizes (in base pairs) are given on the left. (C) qRT-PCR analysis of genes surrounding bb0318 in mutant and complemented clones. Spirochete RNA was isolated from log-phase-grown WT, mutant, and complemented clones, and the expression of the recA, bb0319, bb0318, bb0317, and bb0316 genes was quantified by reverse transcriptase qPCR. The data are expressed as the number of mRNA transcripts per number of recA transcripts and are averages for three biological replicates. (D) Analysis of the growth of WT, Δbb0318/pBSV2G, and Δbb0318/pBSV2G bb0318 spirochetes. Clones were grown in 5-ml biological triplicate cultures and were enumerated by dark-field microscopy every 24 h.

Article Snippet: A DNA fragment containing the bb0318 gene and an additional 362 bp of upstream sequence containing the putative endogenous promoter was amplified from B31 A3 genomic DNA using the Phusion enzyme (Thermo Scientific) and primers 2088 and 2089.

Techniques: In Vitro, Sequencing, Expressing, Mutagenesis, Construct, Quantitative RT-PCR, Clone Assay, Isolation, Reverse Transcription, Microscopy